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This book will help researchers to understand and develop designer antibodies. It provides a practical overview on how to use state-of-the-art technologies in antibody engineering, for the production of recombinant human or mouse monoclonal antibodies. Subjects such as antibody structure relevant to antibody engineering, plant and mammalian expression vectors and hosts, recombinatorial DNA libraries, PCR cloning of single cells, engineering of affinity and biological effector functions, are thoroughly explored by some of the leading experts in each field.