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The chapters in this book were selected as the best presentations from the 2011 and 2012 annual meetings of the Biochemical Technology division of the American Chemical Society. A panel of industrial and academic membershelped select the content of this volume, along with nominations from individual session chairs. The Biochemical Technology (BIOT) division brings together multiple disciplines, including biotechnology, biochemistry and microbiology. The division has always sought to provide a balanced representation of current research in industry and academia. The chapters in this volume are a reflection of this.This volume brings together cutting-edge research in the areas of upstream and downstream process development, biofuels and biosynthesis. With an emphasis on current challenges facing the bioprocess community, this book contains innovative research by both academics and members of industry. A number of case studies from the biopharmaceutical industry are presented, including practical examples of methodologies for enhanced process development. In addition, the application of new and emergingtechnologies in the fields of biotechnology and bioprocessing are discussed.This symposium book was intended to bring the best of the annual BIOT meetings to a greater audience.