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This book constitutes the refereed proceedings of the 22nd International Conference on DNA Computing and Molecular Programming, DNA 22, held Munich, Germany, in September 16 The 11 full papers presented together with 10 invited and tutorial talks were carefully selected from 55 submissions Research in DNA computing and molecular programming draws together mathematics, computer science, physics, chemistry, biology, and nanotechnology to address the analysis, design, and synthesis of information-based molecular systems