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•This book endeavors to convey knowledge of the time-old principles of biochemistry supplemented with the modern developments in a simple, precise and lucid manner. •The whole subject has been written as per the syllabus of INC, has been divided into 19 chapters, including a chapter on sample collection and reference values.•The content has been thoroughly updated so as to give a recent concept of subject to the nursing students, including the significance of biochemistry in this field.•The chapters are well illustrated with easy-to-reproduce figures and tables. Keeping in mind the fact that the entire syllabus of biochemistry has to be covered in just 30 lectures, only the most essential chemical structures have been elucidated.•Clinical correlation boxes have been added at various places in the text to help the students in understanding the clinical relevance of the subject.•Important terms have been highlighted in all the chapters.