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The main objective of this laboratory manual is to provide the students and academicians with a well-organized, user-friendly and easier tool to better enable them to understand laboratory aspects in the field of Immunology, Animal Biotechnology, and Pharmaceutical Biotechnology. The comprehensive practical training plays a pivotal role for any student studying Life Science. This laboratory manual is the outcome of hands of experience of authors during their research activities as well as teaching experience. This laboratory manual highlights the rules and regulations followed in the laboratory, general methods for waste disposal, laboratory hazards and its first aids, procedure for documentation of lab reports, concepts, protocols, and terminologies used. So, this will help the students to prepare for their oral examinations. The book has been organized into three chapters dealing with various topics in the Immunology, Animal Biotechnology, and Pharmaceutical Biotechnology.