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A protein hormone, begins to be produced by placenta in large amounts in early pregnancy. Detection of HCG confirms the pregnancy. 1. Collect 1st Urine of the morning in a clean, dry and contaminant free plastic or glass container. 2. Use a plastic DROPPER to collect the urine from the container. 3. Remove the test from packaging just before starting the test. 4. Place 2 drops of urine in the sample well/cavity on the test kit. 5. Result appear within 5 Minutes, wait for 5 Minutes. 6. See the Red line on test kit for result POSITIVE or NEGATIVE. 7. Discard the device after use. Result: 1) 2 Pink lines at C & T : Result is Positive 2)1 Pink Line at C : Result is Negative, 3) No Line or 1 Line at T : Result is Invalid. NOTE: 1 kit for one time test only