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This book covers the most important theoretical and practical aspects of the application of HPLC to the biosciences, including sample preparation, column selection, detection of biomolecules, preparative HPLC, and the use of HPLC for specific biomolecules. Finally, the technique is compared to alternative bioseparation methods. The ten chapters are written by researchers active in the biotechnology field. High Performance Liquid Chromatography: Principles and Methods in Biotechnology will be of great practical use to molecular biologists, biochemists and biotechnologists who use HPLC to purify and quantify biomolecules. It will also be of interest to those carrying out protein and DNA characterization and analysis. Table of Contents Introduction to Chromatography in Biotechnology The Principles of Separation by High Performance Liquid Chromatography Column Selection in High Performance Liquid Chromatography Detection and Identification in Biochromatography Sample Preparation The Preparative HPLC of Biomolecules The Application of HPLC for Nucleic Acid Analysis High-pH Anion Exchange Chromatography of Recombinant Glycoprotein Glycans The Application of HPLC for Proteins Alternative Bioseparation Techniques Index