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Written by the best researchers in the field, this timely book explores the use of high pressure in connection with spectroscopy as a research tool in biophysics and biochemistry. It covers subjects such as pressure tunning spectroscopy for investigating molecular interactions; high-pressure FT-IR studies of the secondary structure of proteins; structural, functional, and kinetic aspects of nucleic acid-protein complexes with pressure; sequence, salt, charge, and thestability of DNA at high pressure; steady-state enzyme kinetics at high pressure; membrane-free volume with bulky lipid concentration by regular distribution; and pressure and temperature induced inactivation of microorganisms.