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This book describes a new family of bio-polymergels made from cytoskeletal proteins - actin, microtubule, andtropomyosin. The importance of the gelstate with multi-scale hierarchical structure is emphasized to utilize emergentfunctions in living organisms. Detailed protocol of gel preparation, specifiedmethod of structure investigation, and dynamic studies of self-organization,self-healing, synchronized oscillating, and autonomous motility functions areintroduced together with biomimeticfunctions of synthetic hydrogels.