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The SP-10 Male Fertility Rapid Test Cassette detects Acrosomal Protein SP-10 found on sperms.Since, SP-10 is a protein specific to male germ cells and cannot be found in other cells. This testis very specific for sperms and is now being used for estimating sperm concentration in semen asan aid in determining causes of infertility. The SP-10 Male Fertility Rapid Test detects SP-10gives a normal result, when sperm concentration is above 15 million/mL in semen – a levelinternationally accepted as the minimum level of sperms for normal fertility.Because low sperm count is a leading causeof male infertility, an important first step in determining the cause of infertility is to test the spermcount.Before testing, read the instructions completely carefully and completely.1. Remove the test cassette from the foil pouch and lay it horizontally on a flat surface.2. A semen sample should be collected in the collection cup provided.3. The sample should then be allowed to stand for 60 minutes, until the semen is fully liquefied.4. Using the semen transfer device provided, fill the Semen transfer device up to 0.1 mL indicated on thedevice with the semen sample. The semen sample is then added to the tube of sample dilution bufferprovided.5. Mix the semen sample and sample dilution buffer by turning the tube upside down 5-10 times.6. Hold the diluted specimen buffer tube upright and open the cap onto the specimen collectiontube. Invert the diluted specimen buffer tube and transfer 2 full drops of the dilutedspecimen (approximately 80 μL) to the specimen well (S) of the test cassette, then start thetimer. Avoid trapping air bubbles in the specimen well (S). See illustration below.7. Read results at 5 minutes after dispensing the specimen. Do not read results after 10 minutes