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This Lab Manual of Pharmacology-I has been meticulously prepared in accordance with the latest guidelines prescribed by the Pharmacy Council of India (PCI) for the B. Pharm Second Year, Semester IV curriculum. It is designed to provide students with a comprehensive and practical understanding of fundamental pharmacological principles, experimental procedures, and techniques that are essential for grasping the real-time applications of drugs and their effects on biological systems. Pharmacology, being a dynamic and ever-evolving discipline, bridges the gap between basic medical sciences and clinical practice. Through this manual, students will gain hands-on experience in simulating drug responses using appropriate models, observing pharmacodynamics and pharmacokinetic behaviors, and interpreting the results in a scientific manner. Each experiment in this manual is presented with clear objectives, detailed requirements, step-by-step procedures, observation tables, and relevant theoretical background to reinforce the concepts being studied. This manual serves not only as a tool for performing experiments but also as a guide to understanding ethical considerations in animal experimentation, the importance of precision in laboratory work, and the need for proper data analysis and documentation. Great care has been taken to align the experiments with the core topics covered in the semester, making this manual a useful companion for both theory and practical learning.