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This practical laboratory manual has been designed to provide the exposure to students with methods and protocols in the fields of Molecular Biology, Genetics, Recombinant DNA Technology and Structural Biology. It is a complete instruction manual that imparts knowledge on principles, protocols, observation and conclusions. The fundamentals in this manual are critical to the success of undergraduate/postgraduate students and research scholars. The manual impart students with the basic laboratory skills and knowledge to pursue a career in biotechnology. These exercises and activities serve to engage students and help them understand the fundamentals of working in a biotechnology laboratory. Building students’ skills through an organized and systematic presentation of materials, procedures, and tasks, the manual will help students explore overarching themes that relate to all biotechnology workplaces.Building students’ skills through an organized and systematic presentation of materials, procedures, and tasks, the manual will help students explore overarching themes that relate to all biotechnology workplaces.