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Medical treatment is nowadays driven by evidence. To derive reliable evidence, well planned and carefully carried out studies with appropriate analysis and interpretations are necessary. In this regard, bio statistical methods are the recognized tools. Hence, training of medical and public health student and researchers invariably includes bio statistical methods. The second edition of this book is aimed at providing updated and accurate information on relevant topics. It will serve as a textbook of bio statistics for both graduate and postgraduate medical courses, public health and epidemiology courses and other allied health science courses such as bio statistics, pharmacology, health administration, nursing, and clinical research.