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Written for researchers with an interest in the mechanism of solute transport across biological membranes, this work ranges from the techniques required to study transport itself, through the expression, purification and reconstitution of transporters, to techniques for investigation of their structures. It provides the technical grounding for newcomers to the field, but should also be of value to "old-hands" wishing to get up to date with new-millennium developments in these areas. While some of the approaches described require sophisticated equipment (such as a stopped-flow fluorimeter), most of the protocols can be implemented in any well-found laboratory.