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Methods in Biotechnology and Bioengineering is a self-contained compilation of laboratory experimental protocols to meet the need of students experienced researchers and newcomers to the biotechnology discipline with diversified biotechnological interests. It provides highly useful experimental details on immobilization technology cell and tissue culture electrophoresis conjugation techniques fermentation enzyme isolation and characterization DNA/RNA isolation cloning and analysis hybridoma technology immunological techniques. The protocols are contained in different relevant chapters. Every chapter starts with concise and most relevant introduction to the chapter followed by algorithm of various experimental methods. Methods are very well developed and tested in laboratories from all over the world and presented in this volume with adaptable modifications to make them easily workable. Emphasis has been placed on qualitative procedure central strategy and alternatives to the methods. Also at the end index for at-a-glance accessibility is provided. Hope this book will be a highly helpful instruction guide for self-training of the students in the laboratory without requiring any instructive and demonstrative helps. About the Author Prof. S.P.Vyas has nearly 22 years of research and teaching experience at both U.G. and PG. levels. He has worked as Manager R&D (New Drug Delivery) in Lupin Laboratories Aurangabad for a period of nearly one year. He is a leading scientist and well-known academician of international repute. Prof. Vyas did his postdoctoral research at the School of Pharmacy University of London (U.K.) under the supervision of Prof. Gregory Gregoriadis a renowned liposomologist.He has been awarded Pt. Lajja Shankar Jha Award for excellence in research in Engineering and Technology by the Govt. of Madhya Pradesh. He is a recipient of Motan Devi Dandiya Oration Award 1998 and recently he has been honoured with Best Pharmacy Teacher of the Year 2001 Award by APTI. He has written many invited review articles in Advanced Drug Delivery Reviews Critical Reviews TM in Therapeutic Drug Carrier Systems Pharmaceutical Research and International Journal of Medicine and written many Chapters in national and international books. Dr.D.V.Kohli has nearly twenty two years of teaching experience both at undergraduate and postgraduate levels. He has vast and variegated experience in teaching and research. He is a well renowned scientist who has published over forty research papers in journals of international and national repute. He has been awarded the IDMA research award for the year 1982. He has participated in various national and international conferences as resource person. He is a life member of IPA MPPGA and APTI. His special research interest is in biochemical biotechnological and synthetic bioanalogues.