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Advances in molecular biology and biotechnology are increasing at a rapid pace, both in the development of new methodologies and in their practical applications. This popular textbook has been revised and updated to provide an overview of this exciting area of bioscience and to reflect a number of the key developments driving this expansion. Chapters on the basic methods of key technologies such as nucleic acid analysis and bioinformatics are presented, in addition to genomics and proteomics, which highlight the impact of molecular biology and biotechnology. New chapters on important and emerging methods have been introduced such as gene editing, next generation sequencing, nanobiotechnology and molecular modelling. The first six chapters deal with the core technology used in current molecular biology and biotechnology. These primarily deal with basic molecular biology methods such as PCR, cloning genes and genomes, protein analysis techniques and recombinant protein production. Later chapters address major advances in the applications of specialist areas of molecular biotechnology. Experienced lecturers and researchers have written each chapter and the information is presented in an easily assimilated form. This book makes an ideal text for undergraduates studying these areas and will be of particular interest to students in many areas of biosciences, biology and chemistry. In addition, it will appeal to postgraduates and other scientific workers who need a sound introduction to this ever rapidly advancing and expanding area.