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The field of molecular biology continues to be the most exciting and dynamic area of science and is predicted to dominate the 21st century. Only at the molecular level, it is possible to understand various biological processes in detail. Such understanding is vital for advances in medicine, and the pharmaceutical industry that produces new drugs and thus cure is greatly dependent upon molecular biology. Molecular biology also contributes to our understanding of what human beings are and how they fit into this universe. The objective of this manual is to train young teachers from colleges and research institutions so that methodologies could be used by them in their research in various fields of biology.