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How To Grow Grape From Seed? 1. After dropping the seeds into a container of water, allow them to soak for 24 hours, skimming off and discarding all of them that donâÂÂt sink. 2. Drain the remaining seeds the following day, and place them inside a small container accompanied by a little damp peat moss. One tablespoon of peat moss should be adequate for up to 50 seeds. After capping the container, leave it in a 35- to 40-degrees Fahrenheit refrigerator for three months. 3. At the end of that time period, open the container and plant the seeds at a depth about three times their length in a flat of damp and sterile seed-starting mix. Ensure the flat has bottom drainage holes, and space the seeds 1 1/2 inches apart from each other. Place the flat under a grow light that runs for 16 hours per day because grape seeds germinate best under long-day conditions, and keep the seed-starting mix damp. If the temperature remains at about 70 F during the day, preferably dropping to 60 F at night, some seeds may germinate, or sprout, within two weeks, though others may take two months or longer to sprout. 4. When the little grapevine seedlings have two sets of leaves, transplant each plant into its own 4-inch-diameter pot filled with a mixture that is 8 parts potting soil, 1 part pumice and 1 part sand. For the first month, mix 1 tablespoon of 2-3-1 fertilizer with 1 gallon of water. Afterward, raise the fertilizer amount to 2 tablespoons per 1 gallon of water. Transplant each seedling into its own 1-gallon pot about six weeks after each seedling was moved into the 4-inch-diameter pots. The young grapevines should be ready to go outdoors when each plant has added six true leaves to its original two leaves, which are seed leaves.