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This book disseminates information on paper-based diagnostics devices and describes novel paper materials, fabrication techniques, and Basic Paper-based microfluidics/electronics theory. The section on sample preparation, paper-based electronics/sensors for developing paper-based point-of-care (POC) systems also contains detailed descriptions. In the application sections this book covers sensing technique for DNA/RNA, bacteria/virus and integration of lateral flow assay. The book provides deep understanding and knowledge of paper-based diagnostic device development in terms of concept, materials, fabrication and applications.