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"Practical Applications of Intelligent Systems" presents selected papers from the 2013 International Conference on Intelligent Systems and Knowledge Engineering (ISKE2013). The aim of this conference is to bring together experts from different expertise areas to discuss the state-of-the-art in Intelligent Systems and Knowledge Engineering, and to present new research results and perspectives on future development. The topics in this volume include, but are not limited to: Intelligent Game, Intelligent Multimedia, Business Intelligence, Intelligent Bioinformatics Systems, Intelligent Healthcare Systems, User Interfaces and Human Computer Interaction, Knowledge-based Software Engineering, Social Issues of Knowledge Engineering, etc. The proceedings are benefit for both researchers and practitioners who want to learn more about the current practice, experience and promising new ideas in the broad area of intelligent systems and knowledge engineering. Dr. Zhenkun Wen is a Professor at the College of Computer and Software Engineering, Shenzhen University, China. Dr. Tianrui Li is a Professor at the School of Information Science and Technology, Southwest Jiaotong University, Xi'an, China.