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This text details the expression of cloned DNA or RNA templates in all the major "in vivo" and "in vitro" systems. The "in vivo" systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes and prokaryotes are described, including the prokaryotic systems that offer copuled transcription translation. There is also a chapter on monitoring protein expression. This book is intended for any biomedical researcher wanting to investigate protein expression in cell free systems, viruses, prokaryotic cells, and eukaryotic cells.