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Delighted to present our collaborative effort in publishing a book on Pharmaceutical Biotechnology, I, as the first author, emphasize its research-based nature. Meticulously crafted, the guide aims to help students comprehend Immobilized enzymes' significance, understand Genetic engineering in pharmaceuticals, recognize Monoclonal antibodies' importance, and appreciate microorganisms in fermentation. Structured units begin with a brief biotechnology introduction, moving into enzyme biotechnology, biosensors, and protein engineering. Genetic engineering principles, cloning vectors, and applications in medicine follow. The guide explores immunity intricacies, bacterial vaccines, and hybridoma technology. Further, it covers immuno blotting, genetic organization, microbial genetics, and microbial biotransformation. Fermentation methods and large-scale production conclude the guide. Our goal is to empower students with a holistic understanding of biotechnology's applications, fostering appreciation for its role in pharmaceutical sciences. This guide serves as an essential academic companion, inspiring curiosity in the transformative potential of biotechnology