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Chromatography, a versatile separation technique, involves the separation of mixtures into individual components based on their differential migration rates. Various chromatographic techniques such as high-performance liquid chromatography (HPLC), gas chromatography (GC), and thin-layer chromatography (TLC) are extensively employed in the standardization of herbal products. These techniques facilitate the identification and quantification of active constituents, ensuring the consistency and quality of herbal formulations. By analyzing the unique chemical profiles of herbs, chromatography contributes significantly to the authentication and quality control processes, aligning with regulatory standards and consumer safety expectations in the herbal medicine industry.