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Text Book of Pharmaceutical Biotechnology” provides a thorough introduction to the application of biotechnology in pharmaceutical sciences. It begins with foundational topics such as enzyme biotechnology, protein engineering, genetic engineering, and recombinant DNA technology, explaining their role in modern drug development, diagnostics, and the production of biologics like insulin and monoclonal antibodies. Key areas covered include immunology, vaccine development, and microbial genetics. The book discusses various types of immunity, structure of immunoglobulins, vaccine preparation, and hybridoma technology. It also covers genetic mechanisms like transformation and conjugation, along with microbial biotransformation and fermentation processes. The industrial production of biopharmaceuticals such as antibiotics, vitamins, and therapeutic proteins is explained with practical releva.