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This detailed volume explores methods currently used to investigate the cell wall of various bacterial species and pathogens. By using a combination of genetic, molecular, biochemical, and cytological techniques, the protocols address many fundamental questions involving the composition, biosynthesis, and regulation of bacterial peptidoglycan. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, as well as tips for troubleshooting and avoiding known pitfalls. Authoritative and practical, The Bacterial Cell Wall: Methods and Protocols provides current and future researchers with a compilation of many of the most important and useful procedures in a single resource.