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Microbial infections have a large impact on public health. New species and new variants of microbes continue to be discovered, particularly as we intrude into new ecosystems. Numerous factors clue to the development of Microbial infection and diseases. All are dependent on antibiotics for the treatment of infectious diseases and they are critical for the success of advanced surgical procedures due to microbial resistance. Antibiotic-resistance principles and mechanisms create a massive clinical and financial burden on healthcare systems worldwide. Despite the problem of antibiotic resistance in infectious microbes, little is should be known about the chemotherapeutic agents. So this Book guides a quick review of general chemotherapeutic agents and covers source, Classification, Mechanism, ADRs, contraindications, and uses of it. It’s often fascinating to have a little bit of insight into the textbook where we initially had an idea for this book.