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This is an endeavor to make the graduate students acquainted with the basic knowledge of biotechnology. The book has been written to meet the requirements of B.tech 1st year students (all branches) studying in various engineering colleges affiliated to K.U. Kurukshetra and M.D.U, Rohtak. The book also covers some part of the syllabus of B.Sc. course, and will be of immense help for degree college students. It has been designed to disseminate key information on various aspects of cell biology, genetics, recombinant DNA technology, application of biotechnology in various fields and role of biology in allied fields. The book is divided into 4 units containing 8 chapters in total; each chapter is ended with some selected questions.