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PREFACE TO THE SECOND EDITION An Indian medical graduate should have a sound knowledge of Biochemistry, which includes a systematic study of various biochemical processes associated with health and disease & the analysis of constituents of body fluids or tissue fluids to facilitate diagnosis & prognosis of various diseases. This Competency Based Practical Biochemistry Textbook has been prepared as per the the Competency based undergraduate curriculum for Indian medical graduate prescribed by the Medical Council of India. The cardinal concepts are translated from bench to bed side to suit the undergraduate learning. It emphasizes on competency based undergraduate curriculum with a special focus on early clinical exposure by including guidelines to the students in the laboratory regarding experiments, Quantitative experiments, Demonstrations & Case studies/Case based learning. It also elaborates upon vital topic Objective Structured Practical Examination (OSPE) and its types: unobserved type, response type and performance type. This shall expedite the thorough understanding of practical and clinical aspects of Biochemistry in Medicine by medical students. I express my sincere gratitude to Dr Manjunath KS, Director cum Dean, Professor & HOD of Orthopedics, Bowring & Lady Curzon Medical College and Research Institute, Bengaluru for his magnanimity and motivation in this regard. I am obliged to Mr Khaja Mohideen, Co-ordinator, Dr Meenakshi Parthasarathy, Professor & HOD of Anatomy, Dr Srinivasulu Naidu, Professsor and HOD of Physiology and other colleagues at Bowring & Lady Curzon Medical College and Research Institute, Bengaluru for their tremendous inspiration and also to Dr Sheela HR, Assistant Professor of Pharmacology, Akash Institute of Medical Sciences & Research Centre, Bangalore for her prompt & humongous help. I am deeply indebted to Dr Roopa P Kulkarni, Mrs Anitha D'Souza and Mrs Ramya H Udupa for their support. I, along with my co-authors Dr Kulkarni Sweta and Dr Nimmy Maria James, owe our sincere thanks to our respective parents and spouses for their undeterred moral support. I acknowledge the publishing team of Paras Medical Publisher, for their sincere efforts and co-operation.