3 Review(s)

The topic of "DNA Sequencing Methodologies" is exclusive to the fields of genetics and molecular biology. The several approaches and procedures used to ascertain the precise nucleotide sequence of a DNA molecule are highlighted in the book. Numerous scientific endeavours, such as forensic analysis, medical diagnosis, evolutionary biology, and genomic research, depend heavily on DNA sequencing. Overview of the book's DNA sequencing section: a review of the historical development of DNA sequencing technologies, from primitive manual techniques to contemporary high-throughput systems. A description of the basic ideas of DNA sequencing, including the chemical processes and nucleotide sequence detection. Traditional DNA sequencing methods, including Maxam-Gilbert and Sanger sequencing, as well as the chemical degradation process created by Allan Maxam and Walter Gilbert and an outline of its benefits, drawbacks, and uses. Technologies for next-generation sequencing (NGS) introduction of several next-generation sequencing platforms, including Oxford Nanopore Technologies, Illumina, Ion Torrent, and Pacific Biosciences (PacBio). Text on new developments in DNA sequencing technologies, including benefits and drawbacks, variant calling, and sequence assembly. Information on subjects like genomics, clinical diagnostics, forensic analysis, environmental and microbial genomics, etc., is also included in the use of DNA sequencing. An extensive grasp of the concepts, methods, uses, and difficulties related to DNA sequencing is given in this work. Researchers, students, practitioners, and professionals working in the fields of genetics, molecular biology, biotechnology, and bioinformatics will find it to be an invaluable resource.