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This book provides an overview of single-cellisolation, separation, injection,lysis and dynamics analysis as well as a study of their heterogeneity usingdifferent miniaturized devices. As an important part of single-cell analysis, differenttechniques including electroporation,microinjection, optical trapping, optoporation, rapid electrokinetic patterningand optoelectronictweezers are described in detail. It presents different fluidicsystems (e.g. continuous micro/nano-fluidic devices, microfluidic cytometry)and their integration with sensor technology, optical and hydrodynamic stretchers etc.,and demonstrates the applications of single-cell analysis in systems biology,proteomics, genomics, epigenomics, cancer transcriptomics, metabolomics,biomedicine and drug delivery systems. It also discusses the future challengesfor single-cell analysis, including the advantages and limitations. This book is enjoyable reading material whileat the same time providing essential information to scientists in academia andprofessionals in industry working on different aspects of single-cellanalysis. Dr. Fan-Gang Tseng is a Distinguished Professor ofEngineering and System Science atthe National Tsing Hua University, Taiwan. Dr. Tuhin Subhra Santra is a Research Associate at the California Nano Systems Institute, University of California at Los Angeles, USA.