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Welcome to Microbiology. Microbiology is today accepted as a separate subject and is taught all theundergraduate and postgraduate levels in all Universities and Schools in the World. The present bookhas been written as a course material that can be adopted both at undergraduate and postgraduatelevel. This book is useful to students. At the end of the some chapter has been added referenceswhich help the interested reader.The book comprises ten chapters. The first chapter gives an introduction and discovery of themicrobial world. The second chapters introduces microbial taxonomy. The third chapters describes theeukaryotic cells organelles locations and its functions. The fourth chapters describes variousmicroscopic tools and techniques. The fifth and sixth chapters describes Bacteria and Archaearespectively. The chaper seventh describes basic idea about conjugation, transformation,transduction, and plasmid. The eight and ninth chapters discusses viruses and mycoplasmarespectively. The tenth chapter has a special mention on restriction enzyme, polymerase chainreaction, southern blotting, agarose gel electrophoresis.