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Table of Contents 1. Plant Tissue Culture: An Overview, 2. History of Plant Tissue and Cell Culture, 3. General Methodology of Plant Tissue culture, 4. Culture Media Ingredients, Preparation and Related Problems, 5. Methods of Sterilization and Disinfestation, 6. Aspect Techniques and Preparation of Explants, 7. Histological and Photographic Techniques for Plant Tissue culture, 8. Protoplast Technology, 9. Micropropagation in Plants, 10. Micropropagation: Commercial Laboratory Production, 11. Somatic Embryogenesis Principles, Concepts and Applications, 12. Somaclonal and Gametoclonal Variant Selection, 13. Synseeds or Synthetic Seeds, 14. Plant Tissue Culture and Germplasm Conservation, 15. Plant Tissue Culture and Secondary Metabolite Production, 16. Indexing for Plant Pathogens, 17. Plant Tissue Techniques for Crop Improvement, 18. Plant Tissue Culture Some Related Aspects, 19. Glossary, 20. References