3 Review(s)

Table of Contents Getting Started. Basics of Separation. Detection Sensitivity and Selectivity. Sample Preparation. The Column. Non-Ionic Samples: Reversed- and Normal-Phase HPLC. Ionic Samples: Reversed-Phase, Ion-Pair, and Ion-Exchange HPLC. Gradient Elution. Systematic Approach to the Reversed-Phase Separation of Regular Samples. Computer-Assisted Method Development. Biochemical Samples: Proteins, Nucleic Acids, Carbohydrates, and Related Compounds. Chiral Separations. Preparative HPLC Separation. Quantitation (Including Trace Analysis). Completing the Method: Validation and Transfer. Appendices. Index.