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This volume is a collection ofresearch methods, techniques, and approaches to investigate the molecularbiology of West Nile Virus (WNV). Chapters in the book cover many facets ofWNV, such as propagation and titration of WNV in vero cells, examination of WNVneuroinvasion and neuropathogenesis in the central nervous system of a murinemodel, field surveillance methods for WNV, and in vitro and in vivoblood-brain barrier models to study WNV pathogenesis. A brief introduction,along with a discussion on WNV laboratory safety are also included. Written inthe highly successful Methods in Molecular Biology series format,chapters includes introduction to their respective topics, lists of thenecessary materials and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, West Nile Virus will interest virologists,molecular biologists, microbiologists, and other scientists working with WNV.This book is a comprehensive guide for those conducting research in the fieldof WNV biology, including life cycles, pathogenesis, impact of infection, andpossible treatment/prevention development.